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KMID : 0545119930030010039
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 1 p.39 ~ p.45
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Effect of Various Factors on the Operational Stability of ImmobiIized Cells for Acrylamide Production in a Packed Bed Reactor
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Lee, Cheo Young
Choi, Sang Kyo/Chang, Ho Nam
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Abstract
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The effect of concentrations of phosphate buffer and acrylonitrile, pH, and various salts on the operational stability of the immobilized cells of Brevibacterium CH2 in a packed bed reactor were investigated. The effects of salts and carriers on the swelling of the immobilized beads during hydrolysis in a column reactor were also investigated. Immobilization of the cells in Ba-alginate was more desirable than those in polyacrylamide and Ca-alinate for the swelling of the immobilized beads and the desired quality of the acrylamide produced. High quality acrylamide was produced using the Ba-alginate beads in a recycle fed-batch reactor without using an isotonic substrate. The conversion yield was nearly 100%, including a trace amount of acrylic acid produced as a by-product.
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